Quantitive micromethods for rapid characterization of immune cells activity in infection and cancerous context
Team: Olivier Theodoly (Inserm) - Pascal Rihet (TAGC)
Octobre 2022 - present | CENTURI Postdoctoral fellow
2017 - 2021 | PhD in Physical Chemistry - Institute of Physical Chemistry, Polish Academy of Sciences Warsaw (Poland)
2013 - 2016 | MSc in Laboratory Medicine - University of Chengdu University of Traditional Chinese Medicine (China)
2009 - 2013 | BSc in Laboratory Medicine - Chengdu University of Traditional Chinese Medicine (China)
About her Postdoctoral project
The diagnosis of immune defaults and the design of immune therapies suffer from a lack of quantitative tools to characterize the multiple and complex steps of immune responses. In particular, fast monitoring of immune cell activation would permit rapid assessment of infectious, autoimmune, or cancerous risks. We recently validated a prototypic method to analyze phenotypically and at single cell level the activation of leukocytes from a drop of patient blood. The method, currently considered for a patent, is based on multiprotein micropatterning with micron size resolution. The generated smart substrates have multiple specific functions towards lymphocytes, such as selection, capture, activation and read-out. Rapidity and automatization of the method, together with access to the kinetics of activation at single cell level are key assets to shed new light on physiopathologic questions and to offer new diagnosis opportunities for acute pathologies. We propose here to develop and apply such innovative tools to the cases of sepsis, malaria and immunotherapy treatments.