About his PhD project

The alphaviral protein nsP1 rearranges the cellular cytoskeleton and takes over the membranes as a first step for the generation of viral membranous organelles. nsP1 membrane binding results in the nsP1 oligomerisation in dodecameric pores which activates its capping activity, essential for viral replication and transcription. This is an essential process where membrane binding, membrane bending, protein oligomerisation and allosteric activation of the enzyme are coupled. We have preliminary data showing nsP1 bending membranes by AFM and binding actin filaments by sedimentation and electron microscopy. This thesis aims to explore the forces, structures and dynamics ruling viral organelle formation by the interplay of actin, cell membranes and nsP1. We will provide a model for understanding the formation of cell membranous organelles.