Mechanics of gastrulation in mammals using a minimal in vitro system
Embryonic stem cells in aggregates are able to self-organize in robust and reproducible manner, and generate patterns that recapitulate early embryogenesis. They provide minimal systems to study processes that are difficult to access in vivo, particularly in mammals. Using such systems, we will investigate how stem cells dynamically self-organize in a multicellular environment to generate axial patterns. The objective of the postdoc project will be to analyze and model the self-organization of stem cell aggregates, exposed to global homogeneous biochemical signals and mechanical constrains. The postdoctoral fellow will focus on movements that mimic those observed during gastrulation. The data will be obtained on mouse embryonic stem cell aggregates of controlled starting size, and exposed to morphogens and using live reporters of key signaling pathways.
Self-organization/ Reaction-diffusion/ Stem cells/ Morphogens/ Tissue mechanics/ Gastrulation